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María Jesús Andrés Otero

Servicio de Bioquímica Clínica del Hospital Clínico Universitario Lozano Blesa. Zaragoza.

Thursday, 28 April 2016

NEW DIAGNOSTIC METHODS IN SCHISTOSOMIASIS

Written by Miguel Ansón Manso | Francisco López Alcutén | María Jesús Andrés Otero, Posted in Volumen 2

NUEVOS MÉTODOS DIAGNÓSTICOS EN ESQUISTOSOMIASIS
Male patient, 23 years old and black, from Af-rica who come to the emergency referral from your primary care physician with hematuria subacute / chronic evolution and lumbar pain. In the analytical highlights eosinophilia (11.7%) and the presence in urine sediment Schistosoma eggs Haematobium is confirmed.

Schistosomiasis is used to group all clinical manifestations caused by different species of the genus Schistosoma, which in its adult stage parasites mainly the portal venous system of man. S. haematobium is trematode blood bladder and produces the bladder or urinary schistosomiasis with hematuria; it can also affect the digestive system, the liver or the lungs of the definitive host.

Monday, 30 April 2018

PREANALYTICS, INTERFERENCES AND BIOLOGICAL FLUIDS

Written by María Jesús Andrés Otero | Mercedes Gálvez Castrillo | Dolores Hernandez Villén, Posted in Volumen 7

PREANALÍTICA, INTERFERENCIAS Y LÍQUIDOS BIOLÓGICOS
Figure 1. A) and B) Cerebrospinal fluid (CSF): red blood cells, leukocytes and artefacts (40x).

Performing the cytological analysis of cerebrospinal fluid (CFS). Microscopic vision with a 40x objective reveals a double layer of circular structures ofsimilar size to red blood cells or larger (≈ 10 - 18 microns), and also loose or grouped rectangular structures (≈ 10 - 20 microns) (figure 1A and 1B). The alarm of the alarm of the cytometer was not triggered.


Sunday, 30 April 2017

SLC7A13, NOVEL HETERODIMERIC AMINO ACID TRANSPORTER (HAT) PROPOSED TO BE RESPONSIBLE FOR A TYPE OF CYSTINURIA

Written by María Jesús Andrés Otero | Miguel Ansón Manso | Juan José Puente-Lanzarote, Posted in Volumen 5

SLC7A13, UN NUEVO TRANSPORTADOR HETEROMÉRICO DE AMINOÁCIDOS (HAT) IMPLICADO EN LA CISTINURIA
Figure 1: a) Model of the heteromeric amino acid transporter (HAT) with the heavy subunit (HSHAT) rBAT (brown) and the light subunit (LSHAT) b0, + AT (dark blue ) on the left. On the right, proposed new HAT model with the rBAT subunits (brown) and the light subunit AGT1 (yellow). b) Schematic representation of the HAT structure; rBAT (brown) heavy unit with a large extracellular domain and a transmembrane domain is bound by a disulfide (S-S) bridge by cysteine residues, to the light subunit b0, + AT (dark blue). The rBAT heavy subunit is a membrane glycoprotein with an extracellular domain and the light subunit b0, + AT is a non-glycosylated protein with 12 transmembrane domains. The molecular structure of   subunit AGT1 has not been determined yet.

Cystinuria is an aminoaciduria caused by the defective transport of cystine and dibasic amino acids. The absence of cystine reabsorption in the proximal renal tubule produces an excess of cystine in the urine that causes the formation of kidney stones very difficult to remove by lithotripsy. There is only preventive therapy based on high fluid intake, alkalinization of the urine and treatment with chelating agents1,2.